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Genotyping data management systems

System

Group/Institution

Contact

Germinate

JHI

Sebastian

MontyDB

Cornell

Francisco

GDM

Cornell

Joel

Breedbase

BTI

Ask Titima

Gigwa

CIRAD

Guilhem

PHG

Cornell

Ask Ed

BCF

Broad Institute

GDR-BIMS (https://github.com/laceysanderson )

Un. of Washington

Dori?

IPK (https://zarr.readthedocs.io/en/stable/)

Patrick

VM allocations

VM Hostname

Status

Server Pool

Assignment

username

cbsugobiizvm20.biohpc.cornell.edu

OFF

cbsugobii09

Germinate

jhi

cbsugobiizvm23.biohpc.cornell.edu

ON

cbsugobii09

GDM

gadm

cbsugobiizvm19.biohpc.cornell.edu

ON

cbsugobii10

Gigwa

gigwa

cbsugobiizvm22.biohpc.cornell.edu

OFF

cbsugobii10

PHG

phg

cbsugobiizvm21.biohpc.cornell.edu

ON

cbsugobii11

Breedbase

breedbase

OFF

cbsugobii11

MontyDB

montydb

Each VM has the following resources:

  • 8 CPUs

  • 64 GB RAM

  • 2 TB SSD

  • /storage mounted volumn

  • /shared_data mounted volumn

Users

Username

User

gadm

system

yaw

dave

francisco

Datasets

Dataset

Format

Location

Maize NAM

CSV

/shared_data/test_data/NAM_HM32/csv

Simulated datasets

polyploid data in VCF

Moira share a dataset - invite to next meeting

indel data

rice high density array

vcf

The Rice High Density Array is : 700K SNPs x ~1500 samples

SNPs only

vcf too

Francisco loaded to Gigwa (own instance) already no problem

http://rs-bt-mccouch4.biotech.cornell.edu/staged_data/CSHL_EVA_Release_HDRA.tar

African rice

https://gigwa.ird.fr/gigwa/?module=AfricanRice

available as vcf

metadata availability?

3,000 rice genomes

too large? 29M SNPs

lettuce Wageningen

Public dataset

vcf

12M markers x 500 accessions

3 vcfs - one SNPs, one indels, one structural variants

40 GBs

https://www.nature.com/articles/s41588-021-00831-0/pub/CNSA/data2/CNP0000335/Other/variation
ftp.cngb.org/pub/CNSA/data2/CNP0000335/Other/variation

Lettuce

hapmap

flapjack

cbsugobiizvm19:/shared_data/test_data/lettuce/
  chr1/
    Lactuca__project1__2021-06-24__1152198variants__FLAPJACK.fjzip
    Lactuca__project1__2021-06-24__1152198variants__HAPMAP.zip
    markerlists.zip
  full/
    Lactuca__project1__2021-06-28__12983735variants__FLAPJACK.fjzip
    Lactuca__project1__2021-06-28__12983735variants__HAPMAP.zip

Actions:

  • presentations on polyploid data
  • user accounts for participants
  • identify benchmarking criteria

Benchmarking suggestions

Start with a SNP dataset - vcf ? - check with Sebastian and Breedbase (Titima)

Gigwa - 10s Ms markers x 1000s samples

Loading times?

Extract times - increasing marker and sample numbers?

Start with overview of features so we can understand better benchmarking

Action items April 29th

All - check can access site and load database - Gigwa still to be loaded to VM. Guilhem can access site but needs a user name

Yaw - update confluence so all participants can edit

Yaw - set up slack channel

Yaw - Have user accounts been set up? Set up and distribute. Need user names for people setting up databases.

Yaw - request VMs are not open for security

Dave to set up training with Liz to learn how to use GDM

Yaw make sure to invite Moira to next meeting to discuss polyploid data

Invite Breedbase to next meeting

Liz put together a table overview of features that we can all align against

Schedule a demo of each system features for a future meeting

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