2019-05-03 HTPG Requirements Meeting Notes

15 min NextGen Clients needs- Star/Guillaume

Upcoming service ordering for genomic selection

• 3500+/- samples from NRCRI

  • 3500+/- samples from IITA

Next Gen protocol and order instructions for genomic selection

• Existing sampling instruction

  • Update needs

• Submit DNA Isolation Order Form to InterTek

  • Sampling instruction update needed for DNA extraction at InterTek and sequencing at DArT

    • Blank plate layout need to follow genotyping service vendor at sampling stage, not DNA extraction vendor layout

    • Leave G12 and H12 blank so that InterTek do not need to manually move blanks from H12 and H12 before shipping DNA plates to DArT

• Submit genotyping service order form to DArT

  • For short term, still need to submit two forms

    • In the future, clients only need to submit DNA order form, InterTek will submit genotyping order form for clients

15 min New InterTek-DArT service contract-Petra/Frankie, Andrzej

              InterTek and DArT services

Different InterTek services available EiB clients-

Genotyping services offered at InterTek-Rajagaru and Frankie

Petra’s definition of HTPG projects: DNA extraction at InterTek, genotyping at DNA extraction at InterTek

DNA Isolation Order Form

• H11 and H12 as blank for InterTek genotyping services

• G12 and H12 as blanks for DArT genotyping services

• DNA extraction carry out at InterTek

#1 DNA extraction- (DArT format, $2.2 per sample

#2 DNA quantification-

#3 forwarding DNA plates to gentoyping vendors-

• Low density panel genotyping carry out at InterTek-(KASP marker panel), pricing, product/service catalog

#4 Low density genotyping, 10 SNP panel for diagnostic markers/QTL-pricing

#5 Low for 30 SNP panel-pricing

• New InterTek/DArT Contract

DNA extraction at InterTek and gentoyping at DArT

Genotyping services offered at DArT-Andrej

Genotyping carry out at DArT Services (two online submission and one online submission in the future (one order form from users, InterTek will submit on DArT form), billing directly with InterTek for the future)

# QC

# Mid density

# High density 

10 min HTPG requirements coordination plan-Rajaguru/Eng/Hima

Vendor instruction dissemination methods to clients

HTPG clients volume forecasting and sharing with vendor

Client genotyping order and data retrieve plan

Rajararu-India, Eng-Outside India, now transiting to Rajararu

Current HTPG Dissemination Methods-Rajagaru:

1. Order form, instructions

HTPG user email profile-Eng/Rajararu, can be extended for data distribution,

1. Meetings to engage with HTPG users

1. Marker development and validation

1. Invoicing and charging in the order form-HTPG account, NGC charge code

1. Users send samples to InterTek, order form (# plates, ID of plates, # sample IDs) through emails

15 min How the newly formed EiB HTPG Requirement Working Group help?- Star/Hima and everyone

Plan future meeting update objectives, times/frequency

Hosts, invitees, and attendees

Support and exchange slack channels

 Hima Bindu Kudapato set up survey to agree on new meeting series times, frequency