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- name: A required field to describe the sample name. This is usually the name that gets sent to the lab for processing. Note: A unique sample (within a GDM Crop Database) is defined by the unique combination of the project_name, dnasample_name, and dnasample_number so the dnasample_name does not need to be unique within a project or across projects. The dnasample name in a project can also be the same name as the germplasm name. This is often the case for legacy data existing before sample tracking or LIMS systems were in place.
- uuid: A required field for a unique ID that can be used to identify a sample or sub-samples generated from a plot or plant. We advocate the use of a Universal Unique Identifier which is a 128-bit number used to identify information in computer systems eg 123e4567-e89b-12d3-a456-426655440000. Such a uuid will help to identify and track samples generated from across multiple institutes and systems. As long identifiers may not be manageable by vendors, the sample name can be sent to the vendor, and the uuid maintained internally.
- platename: An optional field describing the plate name that the sample is in. This can be a number (1,2,3,4, etc.), or a name given by the lab.
- num: A required field describing the numerical order of the sample within a project. For example, 1-96 for a 96 well plate. The combination of dnasample name and num need to be unique within the project.
- well_col: An optional field describing the plate column coordinates for the sample. For example, 1-12 for a 96 well plate.
- well_row: An optional field describing the plate row coordinates for the sample. For example, A-H for a 96 well plate.
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- Click Next without mapping any terms related to Germplasm and go to DNAsample Information table directly.
- The mandatory fields for both DNASample Information and DNArun/DS_DNArun Information are highlighted with bluish green (cyan) color. The left box shows the terms from your data file.
- Drag and drop your dnasample_name, germplasm_external code, dnasample_UUID, and dnasample_number from Data file fields to name, external_code, number, respectively in the DNAsample Information table.
- If you have information on dnasample_platename, dnasample_well_col, dnasample_well_row are available in your data file, you can drag and drop the information in the same way as shown in the screenshot below:
- Drag and drop your DNArun_name, dnasample_name, dnasample_number from Data file fields to name, dnasample_name, number, respectively in the DNArun/DS_DNArun Information table.
- If you have information on dnasample_platename, dnasample_well_col, dna_well_row are available in your data file, you can drag and drop the information in the same way as shown in the screenshot above.
- Use the Property table which is given below DNAsample Information table and DNArun/DS_DNArun Information table to map the terms related to DNA sample and DNArun properties .
- Drag and drop your additional germplasm fields from Data file fields into the Property field and click Next.
- Skip mapping terms to DNA Sample Information table and just click Next.
- Click Finish to load the germplasm file to the database. You may need to login to log into the server to submit the file.